Common Stock, par value $0.01 per share

Enzo Biochem, Inc.

21 Executive Blvd., Farmingdale, NY, 11735

This Schedule 13D is being filed on behalf of Battery Ventures XIV, L.P. ("BV14"), Battery Ventures XIV-EF, L.P. ("BV14-EF"), Battery Investment Partners XIV, L.P. ("BIP14"), Battery Partners XIV, L.P. ("BP14"), Battery Partners GP XIV, LLC ("BPGP14"), Neeraj Agrawal ("Agrawal"), Michael M. Brown ("Brown"), Morad Elhafed ("Elhafed"), Jesse Feldman ("Feldman"), Russel L. Fleischer ("Fleischer"), Roger H. Lee ("Lee"), Zachary Smotherman ("Smotherman"), Chelsea R. Stoner ("Stoner") and Dharmesh Thakker ("Thakker"). The foregoing individuals are collectively referred to as the "BV Managing Members" and, together with the foregoing entities, the "Reporting Persons." The Reporting Persons disclaim membership in any "group" with any person other than the Reporting Persons. The filing of this Schedule 13D by the Reporting Persons shall not be considered an admission that such Reporting Persons, for the purpose of Section 13(d) of the Exchange Act, are the beneficial owners of any of the shares of common stock covered in this report, and the Reporting Persons expressly disclaim such beneficial ownership. The agreement among the Reporting Persons to file this Schedule 13D jointly in accordance with Rule 13d-1(k) of the Securities Exchange Act of 1934, as amended, (the "Act") is attached hereto as Exhibit 99.1.

The principal business office of the Reporting Persons is One Marina Park Drive, Suite 1100, Boston, MA 02210.

The principal business of the Reporting Persons is venture capital investments. Agrawal, Brown, Elhafed, Feldman, Fleischer, Lee, Smotherman, Stoner and Thakker are the sole managing members of BPGP14, the general partner of BIP14 and BP14, which is the general partner of each of BV14 and BV14-EF.

During the last five years, none of the Reporting Persons has been a party to a civil proceeding of a judicial or administrative body of competent jurisdiction and as a result of such proceeding was or is subject to a judgment, decree of final order enjoining future violations of, or prohibiting or mandating activities subject to, federal or state securities laws or finding any violation with respect to such laws.

Agrawal, Brown, Feldman, Elhafed, Fleischer, Lee, Smotherman, Stoner and Thakker are citizens of the United States. BV14, BV14-EF, BIP14 and BP14 are limited partnerships organized under the laws of the State of Delaware. BPGP14 is a limited liability company organized under the laws of the State of Delaware.

As a result of the Voting Agreements, the Reporting Persons may be deemed to be the beneficial owner of 11,575,824 shares of the Issuer's Common Stock, which includes 656,787 shares of Common Stock issuable upon the exercise of stock options and 79,780 shares of Common Stock issuable upon the settlement of restricted stock units covered by the Voting Agreements that are either exercisable or assumed to be exercisable within 60 days of June 30, 2025. The Reporting Persons may be deemed to have the shared power to direct the vote of the shares of the Issuer's Common Stock subject to the Voting Agreements with respect to those matters described in Item 4. However, the Reporting Persons (i) are not entitled to any rights as a shareholder of the Issuer as to the shares of the Issuer's Common Stock subject to the Voting Agreements, (ii) disclaim any beneficial ownership of the shares of the Issuer's Common Stock covered by the Voting Agreements, (iii) do not have the power to dispose of the shares of the Issuer's Common Stock covered by the Voting Agreements, and (iv) do not have the power to cause the stock options to be exercised. Except as described in this Schedule 13D, none of the Reporting Persons beneficially owns any shares of the Issuer's Common Stock. BP14 is the general partner of each of BV14 and BV14-EF; BPGP14 is the general partner of each of BP14 and BIP14. The BV Managing Members are the managing members of BPGP14.

Rows 7 through 10 of each Reporting Person's cover page to this Schedule 13D set forth the number of shares of Common Stock as to which such Reporting Person has the sole or shared power to vote or direct the vote and sole or shared power to dispose or to direct the disposition and are incorporated by reference.

Except as set forth herein, none of the Reporting Persons has effected any transactions with respect to the securities of the Issuer during the past sixty days.

No other person is known to have the right to receive or the power to direct the receipt of dividends from, or any proceeds from the sale of, the securities beneficially owned by any of the Reporting Persons.

Not applicable.